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SUNRISE 61
PINEAPPLE 29
SCREWDRIVER 168
RAVEN 322
GREENGRASS 430

marilou_262005@hotmail.com

p1083268

https://liars.forumsrpg.com/t2879-quand-le-chat-n-est-pas-la-les-souris-dansent-maya#61664

226-1221

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SUNRISE 61
PINEAPPLE 29
SCREWDRIVER 168
RAVEN 327
GREENGRASS 438

We first verified the effects on worm lifespan of metformin, and also the more potent biguanide drug phenformin. Metformin at 25, 50, and 100 mM increased mean lifespan by 18%, 36%, and 3% (Figure 1A;Table S1available online). Phenformin at 1.5, 3, and 4.5 mM also increased lifespan, by 5%, 21%, and 26% (Figure 1B;Table S1). As expected, maximal effects on lifespan of these pharmacologically similar drugs were non additive Figure 1. The Biguanide Drugs Phenformin and Metformin Decelerate Aging in C. elegans (A and B) Metformin (A) and phenformin (B) extend lifespan in a dose-dependent manner. Phenformin alters fecundity and reduces body size (see Figures S1A–S1D). (C) Phenformin (4.5 mM) does not increase lifespan in the presence of 50 mM metformin, consistent with similar mechanism of drug action (D) Metformin decreases the exponential increase in age-related mortality (for survival curve see Figure S1E). (E) Later-life administration (day of metformin increases lifespan at lower concentrations (25, but not 50 or 100 mM). See also Figure S1. For statistics, see Table S1. (Figure 1C;Table S1). Metformin reduced the exponential age increase in mortality rate (Figure 1D), demonstrating that it slows aging (at least until day 18) rather than reducing risk of death. Metformin also modestly increased mean lifespan when administered from middle age onward, but only at 25 mM (+8%, p < 0.001;Figure 1E;Table S1). In most trials, the DNA replication inhibitor FUdR was used to prevent progeny production, but effects of metformin on lifespan are not FUdR-dependent (Figures S1F and S1G;Table S1). These results confirm the robust effects of biguanide drugs on aging inC. elegans. Interventions altering E. colican affect C. Elegans lifespan. To test the possibility that metformin increases worm lifespan by altering the E. coli, we assessed its effects in the absence of bacteria (axenic culture). As expected, culture on axenic medium (Lenaerts et al., 2008) and bacterial deprivation caused an increase in worm lifespan, typical of DR. Under these conditions, metformin did not increase worm lifespan, but instead markedly reduced it (Figures 2A,S2A, and S2B;Table S2). UV-irradiation of E. Coliim pairs bacterial viability and extends worm lifespan without reducing fertility, suggesting a mechanism distinct from DR. Under these conditions, metformin still shortened lifespan (16%, p < 0.001; Figure 2B;Table S2). Next, we raised E. coliin the presence of metformin and then transferred it to drug-free agar plates. Drug pretreatment ofE. colirobustly extended worm lifespan . We conclude that the life-extending effect of metformin is mediated by live E. coli. Moreover, in the absence of E. coli, metformin shortens C. elegans lifespan, likely reflecting drug toxicity. One possibility is that metformin extends worm lifespan by reducing E. coli pathogenicity. Proliferating E. coli block the alimentary canal in older worms, and antibiotic treatment can both prevent this proliferation and increase worm lifespan (Garigan et al., 2002). To determine whether metformin extends worm lifespan by preventing E. coli proliferation, we tested its effects in the presence of carbenicillin. This antibiotic is bacteriostatic, blocking bacterial proliferation without greatly reducing its viability. Metformin increased lifespan to a similar degree in the absence (+25%) or presence (+24%) of carbenicillin (p < 0.001; Figure 2D;Table S2). Thus, metformin does not increase lifespan by preventing bacterial proliferation. Culture of C. Elegans with Bacillus subtilis increases lifespan (Garsin et al., 2003), suggesting that this microbe is less pathogenic toC. elegansthanE. coli. Metformin increased lifespan of worms cultured on B. subtilis
(+9%, p < 0.001; Figure 2E; Table S2). These findings suggest that reduced bacterial pathogenicity is not the cause of metformin-induced longevity. Biguanides Have Bacteriostatic Effects at Concentrations that Increase Lifespan Biguanides induced a dose-dependent inhibition ofE. coliproliferation (Figures 2F andS2C) and an alteration in bacterial lawn morphology (Figure 2G). Similar results were obtained with B. subtilis(Figures S2D–S2F). Thus, metformin can also act as (Garigan et al., 2002). To determine whether metformin extends worm lifespan by preventingE. coliproliferation, we tested its effects in the presence of carbenicillin. This antibiotic is bacteriostatic, blocking bacterial proliferation without greatly reducing its viability. Metformin increased lifespan to a similar degree in the absence (+25%) or presence (+24%) of carbenicillin (p < 0.001; Figure 2D;Table S2). Thus, metformin does not increase lifespan by preventing bacterial proliferation. Culture ofC. Eleganswith Bacillus subtilis increases lifespan (Garsin et al., 2003), suggesting that this microbe is less pathogenic to C. elegans than E. coli. Metformin increased lifespan of worms cultured on B. subtilis (+9%, p < 0.001; Figure 2E;Table S2). These findings suggest that reduced bacterial pathogenicity is not the cause of metformin-induced longevity. Biguanides Have Bacteriostatic Effects at Concentrations that Increase Lifespan Biguanides induced a dose-dependent inhibition of E. coli proliferation (Figures 2F andS2C) and an alteration in bacterial lawn morphology (Figure 2G). Similar results were obtained with B. subtilis(Figures S2D–S2F). Thus, metformin can also act as (Garigan et al., 2002). To determine whether metformin extends worm lifespan by preventing E. coliproliferation, we tested its effects in the presence of carbenicillin. This antibiotic is bacteriostatic, blocking bacterial proliferation without greatly reducing its viability. Metformin increased lifespan to a similar degree in the absence (+25%) or presence (+24%) of carbenicillin (p < 0.001; Figure 2D;Table S2). Thus, metformin does not increase lifespan by preventing bacterial proliferation. Culture ofC. Elegans with Bacillus subtilis increases lifespan (Garsin et al., 2003), suggesting that this microbe is less pathogenic to C. Elegans than E. coli. Metformin increased lifespan of worms cultured on B. Subtilis (+9%, p < 0.001; Figure 2E;Table S2). These findings suggest that reduced bacterial pathogenicity is not the cause of metformin-induced longevity. Biguanides Have Bacteriostatic Effects at Concentrations that Increase Lifespan Biguanides induced a dose-dependent inhibition of E. coli proliferation (Figures 2F andS2C) and an alteration in bacterial lawn morphology (Figure 2G).

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SUNRISE 61
PINEAPPLE 29
SCREWDRIVER 168
RAVEN 329
GREENGRASS 439

got a secret can you keep it

SUNRISE 61
PINEAPPLE 29
SCREWDRIVER 169
RAVEN 331
GREENGRASS 440

ACROBATES: 0
AMUSEURS PUBLICS: 66
CLAIRVOYANTS: 67
CLOWNS: 0
DOMPTEURS: 6
FORAINS: 1
MAGICIENS: 75
MONSTRES DE FOIRE: 1
STARS ET DIVAS: 0

#cc0000

:

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SUNRISE 61
PINEAPPLE 29
SCREWDRIVER 169
RAVEN 334
GREENGRASS 443

Gamer Poop - Mass Effect 3

SUNRISE 61
PINEAPPLE 29
SCREWDRIVER 169
RAVEN 335
GREENGRASS 444

Chapitre Quatrième
C toi le gars se fume la jambe en criant
tu vois cette petite chose c'est une tentacule, c'est dans les cartes
arhrahrharhqarharharhraharhra
on voit l'estomac de ct oi le gars
asjdhgasdgajshdaasararararara saura dans quelle équipe elle se trouve grace au papier dans la flamme qu'elle allume
on fait des experimentations avec le poulet genre, le fey est bouillant
non je parlais du chcocolat fondu
fumer avec sa jambe, une jambe pourra le lighter chris ne rit pas apres relecture
chriss be like woooooooooow il ne se passe toujours rien
arararara a un oeil dans son poulett
yha un polet dans mon cul

arararara t une poulette girl
loeil de arararar est un ciel dazur dété
tcheck penetration anale kinder
on fait fondre la valise on fait pas fondre la valise


donne moi ton vagin dit ararararar
c toi le gars lui donne son vagin
la fille navairt plus de front fin
la fille se crisse la pipe dans le vagin et en ressort des poussins

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ACROBATES: 0
AMUSEURS PUBLICS: 83
CLAIRVOYANTS: 86
CLOWNS: 0
DOMPTEURS: 6
FORAINS: 1
MAGICIENS: 79
MONSTRES DE FOIRE: 1
STARS ET DIVAS: 0

(Et oui, j'ai rien copié aujourd'hui^^)